Infectious vibrio parahaemolytic real time pcr kIt is utilized for for the detection of vibrio parahaemolytic and internal control ic from shrimps. Vibrio parahaemolytic has been wildly reported as a leading major diseases in shrimp culture industry in Asia's southeast region central america and southern usaIt acts as known to be transmitted both vertically and horizontally. Various such as, Molecular methods microtomy, Immuno, Assay, Hybridization, And pcr polymerase chain reaction for pathogen detection have been developed for shrimp diseases. Real time taqman probe, Based detection the system operates as proven to be highly sensitive and determines the result in real time. For the progression of effective diagnostic tools, Vibrio parahaemolytic genomic library has been constructed and analyzed. In addition, A highly conserved region of vibrio parahaemolytic has furthermore been selected as the target for diagnosis. The technology incorporated in kit is developed at aura biotechnologies private limited.