The tc, 3 bioreactor is crafted to meet specific needs as a simple easy, To, Use system suitable to create cell culture experimental set, Ups applying mechanical loading profiles defined by the perstransitioning to the samples. The needs of researchers working on cell culture under mechanical stimuli have been accounted for inside the layoutside of the tc, 3. It combines the qualities of a traditional testing machine including the distinct demands of cell culture, With special emphasis on the sterilization of the parts which are going to the easy assembling, Contact the the sample, Culture medium inspection by microscopy techniques and the straightforward sample manipulation. Applicationsthe tc, 3 permits to work with horizontally and vertically arranged multiple samples immersed and air, Liquid interface setups sheet, Shaped, Rod, Shaped and cylinder, Shaped substrates tension and compression axial loading simultaneous tension/compression and flow through the substrate and hydrostatic pressurevisual inspection of the samples is provided owing to its transparent lid and bottom glass window. Also during engagements with thin membrane, Shaped scaffolds, It is possible to accomplish to use microscopy techniques to inspect the status of the culture in, Situ. Scalability and versatility are essential attributes of the tc, 3, In which three grips models can be interchanged in an effort to adapt to the features from the evaluationing substrate or scaffold. The system is regulated by a simple computer interface which allows defining the most common loading profiles that is designed to being applied on the culture substrate.