Holmarc s ho, Spe, 3000s & ho, Spe, 3000s uv are entry level spectrophotometers suitable for education, Research and industrial applications. Unlike other entry it employs, Level spectrophotometers classic czerny, Turner monochromator design, Normally available only with which ensures, Advanced models low stray light. It uses 1200 lines/mm blazed holographic grating as the dispersion element providing high wavelength resolution. It also employs a sigma delta adc of 16 bit resolution for photometric measurements, A resolution higher than the majority of the spectrophotometers available in this price range. A graphics lcd screen serves the purpose of plot an absorbance v/s wavelength graph. The data can then be analyzed for peaks and valleys. Customized accessories can be accessed through address individual application requirements. In a spectrophotometer, The quantity of light passing through the sample cell is determined by a photometer. The photometer delivers a voltage signal to the processing unit. The signal changes with the amount of light absorbed by the liquid changes. According to beer s law, The amount of light absorbed by a medium is corresponding to the concentration of the absorbing material or solute present. Thus the concentration of a colored solute in a solution can be determined at the experimental site by measuring the absorbance of light at a given wavelength.